ImageJ Pipeline Builder

Native LLM and Python Integration for ImageJ. Build repeatable automations to save time.

AI Pipeline Builder in ImageJ — chat interface with microscopy images and analysis results

How it works

1

Describe

Open the builder in ImageJ and describe your analysis task in plain English.

2

Refine

The AI understands your intent and asks smart follow-up questions about your image.

3

Test

The AI will generate a script. The model knows how to perform your task, but can make mistakes. Inspect and test your script for accuracy.

4

Save

Save your complete script in the ImageJ menu for convenient access for repeated actions.

AI Pipeline Builder

Welcome! Describe what you need and I'll build it for you.

Build your plugin natively in ImageJ

Natural Language building

Turn "give me the fluorescent intensity of every cell in the image" into a working plugin in 10 seconds.

Python Integration

Bridge ImageJ macros with Python. Use CellPose, scikit-image, or your own models directly from the builder.

Batch Processing

Run your pipeline across entire folders. Process hundreds of images with consistent parameters and automatic export.

Installs Easily

1

Download IJPB Plugin

Grab the latest release.

2

Drag JAR into Fiji

Drop it onto the Fiji window. It installs itself.

3

Restart ImageJ

Relaunch and find the plugin under Pipelines → New

Drag into ImageJ
IJPB.jar
Fiji window
Success! Now you're ready to try it yourself Download Here

Always Free.
Cloud if you need it.

If you have an LLM API key, use it. If you don't, you can use ours. Usage limits apply.

  • Bring a ChatGPT or Claude API Key
  • Ollama support for local LLMs
  • Use our cloud if you don't have an API key.

Free

Build what you need when you need it

Configure with your own API key and build with no limits. No API Key? Use our LLM backend for free.

Get Free

Cloud  $15 / mo

Preconfigured LLM backend for api-key free access

Support our development and gives you expanded access to our AI cloud backend.

Start Cloud

See what others have built

Real pipelines from the IJPB community. Click any card to see the conversation.

Cell Biology

DAPI Nuclei Counter

"Count DAPI-stained nuclei, separate touching cells, and export count and mean area per image."

Segmentation

CellPose Membrane Detector

"Use CellPose to segment HeLa cells in phase contrast and output individual cell mask TIFFs."

Colocalization

GFP/RFP Colocalization Report

"Measure Pearson and Manders coefficients between GFP and RFP channels across 48 images."

FISH

smFISH Spot Counter

"Count mRNA FISH spots per nucleus, normalize to nuclear area, and export a tidy CSV."

Batch Processing

Batch Fluorescence Analyzer

"Rolling-ball background subtraction + mean intensity per channel per ROI across 200 images."

Live Cell

Mitochondria Fission Tracker

"Detect and track mitochondrial fission events in a 100-frame time-lapse using TrackMate."